FastQCFastQC Report
Thu 4 Dec 2014
salivary_repl1_R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamesalivary_repl1_R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100000
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGATGCACCGGCCAGAGGCCATGCTGCTGCTGCTCACGCTTGCCCTC1490.149No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGG504.1758176E-757.0528561
ATCGGGA652.566916E-643.8429222
TTTACCC500.001609351437.99722
GATATCG300.003944644731.66433334-35
CGGGAGA953.4611447E-529.997794
TCGGGAG804.46661E-429.6853143
CGGATCA404.4824582E-429.68531448-49
CGGGGGG705.76958E-727.14085838-39
GAGGGGC1109.396788E-525.907189
GACCTTA652.9312255E-421.92146146-47
GAAACAA1100.00286045621.5893173
TCGGGGG1005.3441363E-721.37342538-39
GTCATTT1353.7390977E-421.1306881
TTCACTT1150.003701481520.6506522
AGGGGCG650.007545068418.26788510-11
TTTGACC1300.00751936918.2678856
AGGGGAG1051.9439336E-518.09390428-29
GGGAGAG1952.2505556E-417.0500265
AGAGGGG1450.004542752616.3781038
GATAACT900.002609767715.83216716-17
Produced by FastQC (version 0.11.2)