FastQCFastQC Report
Thu 4 Dec 2014
salivary_repl1_R2.qc.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamesalivary_repl1_R2.qc.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95236
Sequences flagged as poor quality0
Sequence length25-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGGATGCACCGGCCAGAGGCCATGCTGCTGCTGCTCACGCTTGCCCTC1420.149103280272166No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT502.3588655E-839.06104386-87
CGTCGTG602.8703653E-936.61972890-91
CAGGCGG550.002761586634.036674
GGACATC550.002761586634.036672
GAGGGTC550.002761586634.036679
AGGGTCG352.2333095E-433.4288710-11
ACTTCAC650.006254933328.8002595
AAGAGGT700.00898054726.7430953
GATCTCG350.0090027426.74309534-35
TCGTGTA858.5179636E-825.84921892-93
GCGTCGT500.001404114524.41315388-89
ATCGGAA901.4809302E-724.4131580-81
AGCGTCG952.4963265E-723.12824888-89
CGGAAGA852.4602814E-622.97708382-83
GTGTAGG1003.975474E-722.03825294-95
AGAGATC600.00397862220.4057994-95
GGATTAT600.005096567319.5515250-51
AGATCGG1102.285418E-517.7550278-79
CGTGTAG700.00985959917.43796592-93
GAGATCG1000.004290319514.69216894-95
Produced by FastQC (version 0.11.2)