FastQCFastQC Report
Thu 4 Dec 2014
salivary_repl1_R1.qc.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamesalivary_repl1_R1.qc.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95236
Sequences flagged as poor quality0
Sequence length81-101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGC4220.44310974841446515TruSeq Adapter, Index 10 (100% over 50bp)
CTGGGATGCACCGGCCAGAGGCCATGCTGCTGCTGCTCACGCTTGCCCTC1620.17010374228233022No Hit
GCAAGAGTCATTTTGACCAGCAGATTAATCAACTGTAAGACGGATCCTCA1080.1134024948548868No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTAGG250.005013977656.9804575
ATTTATC459.5967646E-442.207756
CGTATGC501.2951241E-633.238644-45
CTCGTAT501.2951241E-633.238642-43
TATGCCG605.3650892E-627.6988446-47
TTGATCC700.00813418827.2912253
AAAATCC700.00813418827.2912253
CCGTCTT659.990443E-625.56815750-51
TGCTTGA751.0555032E-625.3246558-59
ATCTCGT701.7740862E-523.7418640-41
GTCGAAG500.001651893623.74185812-13
TCGTATG500.001651893623.74185842-43
AATAGCG550.002874365321.58350826-27
GCCATCA1604.7248694E-520.8948443
ATGCCGT704.842499E-420.35016646-47
GCTTGAA600.004758608519.78488558-59
TATCTCG600.004758608519.78488538-39
TGCCGTC857.930337E-519.5521248-49
GCTTATC901.229705E-418.4658936-37
CCATCAA1300.00728071418.3690934
Produced by FastQC (version 0.11.2)