FastQCFastQC Report
Wed 10 Dec 2014
female_repl1_R2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefemale_repl1_R2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTT250.005051161656.8759421
CCCGATC250.005051161656.8759421
TGGCCCG250.00522486156.3924988
CCGTGCT600.003975894331.5977461
AAAGTTC1100.003024997221.3821772
GTCGTGT1751.06635394E-418.797594
GGGGGAT650.00802248818.0745214-15
AGCGTCG2700.002865396212.18356594
GAGCGTC2851.12139554E-411.54232594
GCGTCGT2258.303299E-611.48736192-93
AGAGCGT3051.8220453E-710.78545192-93
GAGATCG2809.246219E-59.23091592-93
CGTCGTG2050.0060056629.16951292-93
CGGAAGA4058.634344E-68.12237688-89
AAGAGCG3254.64175E-47.95278888-89
ATCGGAA4453.0284795E-57.39227586-87
GAAGAGC4757.158563E-56.925394590-91
AGATCGG5203.1836957E-56.77794584-85
Produced by FastQC (version 0.11.2)