FastQCFastQC Report
Wed 10 Dec 2014
female_repl1_R2.qc.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefemale_repl1_R2.qc.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95583
Sequences flagged as poor quality0
Sequence length99-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG502.785525E-547.56223794
GATGCAT555.2533993E-542.7179955
GTCGAGG459.999942E-441.856321
CAGATCG600.003907638631.70815794
TATCGCG300.004156831.32653220-21
TTTGAGA804.6965212E-429.4302252
ATATCGC350.00882727326.8513159
ATGCATC700.00880558726.8513156
ATCGCGG350.00882727326.85131520-21
TGGATGC950.001281727624.7833483
GACTGCC550.003054172321.35899740-41
CTGGATG1300.007900418.1109072
Produced by FastQC (version 0.11.2)