FastQCFastQC Report
Wed 10 Dec 2014
female_repl1_R1.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefemale_repl1_R1.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences100000
Sequences flagged as poor quality0
Sequence length100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC2240.22399999999999998TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTAATC250.005176557756.524877
CGTATGC350.00885306226.83585744-45
CTCATTT1150.003717236520.6352581
CTCGTAT705.2176864E-420.12689242-43
AATATTA650.00805306518.06259570-71
AGCACAC3156.693108E-411.92704894
CTGCTTG1550.00811615910.60449156-57
AGAGCAC3408.1666803E-79.66880192-93
GATCGGA4952.733553E-49.48742594
GAGCACA3500.00841819.3925594
GAGATCG2901.36479E-48.90672992-93
GCACACG2809.473047E-48.38620692-93
CGGAAGA4555.5365854E-78.25718788-89
ATCGGAA4453.0550596E-57.38739986-87
AGATCGG5153.5813755E-67.295184692-93
GAAGAGC5403.8259814E-46.95744494
GGAAGAG5900.0071862576.367830894
TCGGAAG4300.0089478726.00686388-89
Produced by FastQC (version 0.11.2)