FastQCFastQC Report
Wed 10 Dec 2014
female_repl1_R1.qc.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefemale_repl1_R1.qc.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95583
Sequences flagged as poor quality0
Sequence length28-100
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC2230.2333050856323823TruSeq Adapter, Index 7 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGGAAG452.0154039E-763.3877143
CTCCGAT250.00499006857.048941
ATCGGAA504.1753083E-757.048942
CTCTAGT250.00521356256.421376
CTAGTAT451.4976689E-552.8230972
ATGGCCC451.599139E-552.2420167
CGGAAGA601.4689467E-647.5407834
TGGCCCG652.7608148E-643.378338
GATCGGA704.2431166E-640.7492451
TAGTATG608.191493E-539.617323
AGATCGG608.424575E-539.42936394
TATGGCC608.7438624E-539.1815076
ACTAGTA500.00160154538.0326271
GTATGGC651.3706992E-436.300655
TAGCCTG300.004165988431.312432-33
ACGGCAA300.004165988431.312414-15
ACACGTC505.32486E-528.18116212-13
TCTCGTA505.32486E-528.18116240-41
ATGCCGT505.32486E-528.18116246-47
GATAGTA350.00884671626.83920178-79
Produced by FastQC (version 0.11.2)